Epithelial expression of TLR4 is modulated in COPD and by steroids, salmeterol and cigarette smoke-0

<p><b>Copyright information:</b></p><p>Taken from "Epithelial expression of TLR4 is modulated in COPD and by steroids, salmeterol and cigarette smoke"</p><p>http://respiratory-research.com/content/8/1/84</p><p>Respiratory Research 2007;8(1):84-84.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2194695.</p><p></p>g. Subjects were excluded on the basis of pre-existing immunosuppression, pulmonary or nasal pathology other than COPD, including current or recent (within 6 weeks) upper or lower respiratory tract infection. Tracheal brush specimens were also collected on a subset of patients undergoing fibreoptic bronchoscopy (n = 9). A. Table showing demographics of the study population. There was no significant difference between the study groups. B. Total RNA from was reverse transcribed into cDNA and used as a template for semi-quantitative PCR reactions using TLR4, TLR2, HBD2 and GAPDH gene-specific primers. (** P < 0.005 vs non-smoking controls; † P < 0.05 vs all controls; $ P < 0.05 vs COPD FEV1 > 1L). C. TLR4 expression by semi-quantitative RTPCR analysis in tracheal and nasal epithelium