A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosis-6

<p><b>Copyright information:</b></p><p>Taken from "A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosis"</p><p>http://www.biomedcentral.com/1471-2164/8/384</p><p>BMC Genomics 2007;8():384-384.</p><p>Published online 22 Oct 2007</p><p>PMCID:PMC2204013.</p><p></p>e-cocktail-stimulated Mk culture as assayed by flow cytometry. Data is from one representative experiment (n = 3). (B) Cells from cytokine-cocktail-stimulated Mk cultures were co-stained with DAPI (blue), and antibodies against CD41a (red) and GADD45A (green) from day 0 to day 15. Images shown are representative fields (n ≥ 3 per slide). Results are from one representative experiment (n = 3). Image acquired with 20× objective. (C) MAP3K5 (ASK1) protein expression (diamonds) in cytokine-cocktail-stimulated Mk cultures as represented by geometric mean fluorescence intensity of anti-MAP3K5-antibody stained samples relative to cells stained with isotype-matched control antibodies (representative data from n = 2 experiments). Protein expression correlates with average MAP3K5 (ASK1) mRNA expression (squares) in CD41acells as measured by microarray compared to average expression in cytokine-cocktail-stimulated Mk cultures from day 1 – 4 (n = 3). MAP3K5 and GADD45A exhibited similar protein expression patterns in cultures stimulated with Tpo alone (data not shown; n = 2)