Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter-1

<p><b>Copyright information:</b></p><p>Taken from "Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter"</p><p>http://www.aidsrestherapy.com/content/4/1/24</p><p>AIDS Research and Therapy 2007;4():24-24.</p><p>Published online 17 Oct 2007</p><p>PMCID:PMC2211285.</p><p></p>ulation and overnight incubation with Phoenix (GaLV) supernatants. Cells were washed and expanded for 48 hours before analysis by flow cytometry. The dot plots indicate GFP expression versus side scatter characteristics for the empty vector (left plot), 70.15 vector (left center), 70.8 vector (right center), and 80.55 vector (right plot) before sorting. The percentage of GFPcells and the mean fluorescent intensity are indicated in the upper right corner of the plot. B) Challenge of aptamer-transduced CEMx174 cells with HIV-1. Aptamer-transduced CEMx174 cells (empty, filled circle; 70.15, yellow square; 70.8, green diamond; 80.55, blue triangle) were sorted for expression of GFP, infected with HIV-1(MOI = 0.01 TCIDper cell) and followed over 14 days for production of p24 Gag by ELISA