Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer-0

<p><b>Copyright information:</b></p><p>Taken from "Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer"</p><p>http://www.biomedcentral.com/1471-2199/8/107</p><p>BMC Molecular Biology 2007;8():107-107.</p><p>Published online 27 Nov 2007</p><p>PMCID:PMC2211316.</p><p></p>r (Q. = E). Boxplot shows median value, interquartile-range box and outliers (*). Within gene there was no difference in gene quantities between benign and malignant tissues (> 0.05). b) Variation associated with candidate endogenous control genes and in all breast tumours relative to calibrator. Relative gene expression was calculated using the ΔCmethod and corrected for efficiency of amplification (Q. = E). There was a significant difference in variance associated with relative gene expression (= 0.001) with genes such as , , and showing greater variance than genes such as and