IL-23-induced IL-17 production by activated human T cells in a dose-dependent manner

<p><b>Copyright information:</b></p><p>Taken from "IL-23 induces human osteoclastogenesis via IL-17 , and anti-IL-23 antibody attenuates collagen-induced arthritis in rats"</p><p>http://arthritis-research.com/content/9/5/R96</p><p>Arthritis Research & Therapy 2007;9(5):R96-R96.</p><p>Published online 23 Sep 2007</p><p>PMCID:PMC2212562.</p><p></p> Human CD3-positive T cells were cultured in the absence or presence of recombinant human (rh)IL-23 (1.0 or 3.0 ng/ml). After 48 hours, secretion of IL-17 in the cell culture supernatant was assayed by ELISA. Results for one representative donor are presented here and are expressed as means and SD. ELISA measurement of IL-17 production by human CD3-positive T cells treated for 48 hours with various concentrations of rhIL-23 (1.0, 3.0, or 10 ng/ml) in the presence or absence of plate-bound anti-CD3 and anti-CD28, for five representative donors. Data are expressed as means and SD. = 0.009 versus non–activated T cells (Mann–Whitney test). ELISA measurement of IFN-γ production by human CD3-positive T cells treated for 48 hours with various concentrations of rhIL-23 (1.0, 3.0, or 10 ng/ml) in the presence or absence of plate-bound anti-CD3 and anti-CD28, for four representative donors. Data are expressed as means and SD. 0.02 versus non–activated T cells Mann–Whitney test). Ratio of the production levels of IL-17 to those of IFN-γ (IL-17/IFN-γ) calculated from levels of IL-17 or IFN-γ produced from activated CD3-positive T cells at each concentration of rhIL-23 (0, 1, 3, or 10 ng/ml)