Histological appearance of knee joints injected with an adenovirus overexpressing bone morphogenetic protein-2 (BMP-2)

<p><b>Copyright information:</b></p><p>Taken from "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling"</p><p>http://arthritis-research.com/content/9/5/R102</p><p>Arthritis Research & Therapy 2007;9(5):R102-R102.</p><p>Published online 8 Oct 2007</p><p>PMCID:PMC2212581.</p><p></p> Right knee joints were injected intra-articularly with Ad-BMP-2 or a control virus. Mice were injected with SOprior to knee joint isolation for histology on days 3, 7, or 21. Paraffin sections were stained with safranin O/fast green , prepared for autoradiography , and stained immunohistochemically for VDIPEN or NITEGE . Controls displayed here are from day 3 . Cartilage of mice injected with Ad-BMP-2 appeared to have larger chondrocytes than controls . Proteoglycan synthesis had increased by stimulation with BMP-2 . BMP-2 stimulation also leads to increased VDIPEN staining and NITEGE staining . Arrows point to intense staining around chondrocytes. FastG, fast green; SafO, safranin O