The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon-0

<p><b>Copyright information:</b></p><p>Taken from "The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon"</p><p>http://www.virologyj.com/content/4/1/124</p><p>Virology Journal 2007;4():124-124.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2212637.</p><p></p>line, intercistronic region with 5' UTRs of BVDV strain NADL, BVDV strain SD-1, or poliovirus type I. White box, SEAP reporter gene (secreted form of the alkaline phosphatase human placenta); grey box, luciferase reporter gene. The stem-loop structure in construct 3 has a calculated stability of about -73 kcal/mol. Right panel: Relative SEAP and luciferase expression values, levelled out to the specific mRNA content after Northern Blot quantification using a phosphorimager (see Materials and Methods)