Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L-2

<p><b>Copyright information:</b></p><p>Taken from "Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L"</p><p>http://www.biomedcentral.com/1471-213X/7/104</p><p>BMC Developmental Biology 2007;7():104-104.</p><p>Published online 18 Sep 2007</p><p>PMCID:PMC2212652.</p><p></p>ine the expression profile of in primitive type A (PA), type A (A) and type B (B) spermatogonia, preleptotene (PL), leptotene/zygotene (L/Z), prepubertal pachytene (PP) and pachytene (P) spermatocytes, as well as round spermatids (RS) and residual bodies/elongating spermatids (RB). Expression was determined in triplicate in each of the two series of germ cells and normalized to expression; normalized results were calibrated to expression in pachytene spermatocytes. Shown here are the mean expression results obtained for one series. Mean ± SD. Relative quantification of expression in prenatal male germ cells. Quantitative RT-PCR was used to measure the expression levels of in total RNA extracted from E13.5, E15.5 and E18.5 prospermatogonia and 6 dpp primitive type A spermatogonia (PA). Expression of was determined in triplicate in each of the two series of germ cells and normalized to expression; normalized values were calibrated to the expression found in E13.5 gonocytes. Shown here are the mean expression results obtained for one series. Mean ± SD