Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L-3

<p><b>Copyright information:</b></p><p>Taken from "Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L"</p><p>http://www.biomedcentral.com/1471-213X/7/104</p><p>BMC Developmental Biology 2007;7():104-104.</p><p>Published online 18 Sep 2007</p><p>PMCID:PMC2212652.</p><p></p>s. The following tissues were used for analysis: 10 dpp whole testes from and males, and sperm and liver from adult males. Shown here are enlargements of autoradiographs obtained for these tissues. Arrowheads point to five pre-identified germ cell-specific spots present in adult sperm but absent in liver [42]. Densitometry analysis of the five germ cell-specific spots in and testes. Once the somatic-to-germ cell ratio of a 10 dpp testis is considered and the lower germ cell counts in testes are accounted for, the mean density values obtained for and 10 dpp testes are reflective of the proportion of germ cells present in these tissues, not of changes in methylation state. Potential changes in DNA methylation levels in testes fall under the limit of detection