Cell homogenates and CCV fractions were prepared from control and siRNA-treated cells and analysed by Western blot

<p><b>Copyright information:</b></p><p>Taken from "CVAK104 is a Novel Regulator of Clathrin-mediated SNARE Sorting"</p><p></p><p>Traffic (Copenhagen, Denmark) 2007;8(7):893-903.</p><p>Published online 07 Apr 2007</p><p>PMCID:PMC2239300.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> CVAK kd1 and CVAK kd2 denote samples from two independent kd experiments. Approximately 10 μg protein of whole cell lysates or 2.5 μg of CCV fractions were loaded in each lane. Although the four SNAREs, syntaxin 7, syntaxin 8, vti1a, and vti1b, are all sorted by clathrin, only syntaxin 8 and vti1b are depleted from CCVs in a CVAK104 kd. CHC, clathrin heavy chain; kd, knockdown