Effects of siRNA-AdipoR1 transfection on the mineralization of MC3T3-E1 cells

<p><b>Copyright information:</b></p><p>Taken from "Adiponectin and AMP kinase activator stimulate proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells"</p><p>http://www.biomedcentral.com/1471-2121/8/51</p><p>BMC Cell Biology 2007;8():51-51.</p><p>Published online 29 Nov 2007</p><p>PMCID:PMC2214728.</p><p></p> Mineralization of MC3T3-E1 was evaluated by von Kossa and Alizarin red stainings. (A) Plate view of von Kossa and Alizarin red stainings in cultured MC3T3-E1. (B) Quantification of Alizarin red staining via extraction with etylpyridinium chloride. The amount of released dye was quantified by microplate reader at 550 nm. Mineralizarion was significantly decreased in siRNA-AdipoR1 treatment compared to the control (*** p < 0.001). The result was the representative of three different experiments