Bronchial epithelial spheroids: an alternative culture model to investigate epithelium inflammation-mediated COPD-6

<p><b>Copyright information:</b></p><p>Taken from "Bronchial epithelial spheroids: an alternative culture model to investigate epithelium inflammation-mediated COPD"</p><p>http://respiratory-research.com/content/8/1/86</p><p>Respiratory Research 2007;8(1):86-86.</p><p>Published online 26 Nov 2007</p><p>PMCID:PMC2214730.</p><p></p>shows bronchial epithelial sheets-derived spheroids. () Circular pseudostratified epithelium with columnar ciliated cells facing outside, and small pyramidal basal cells. Note, the presence of a central lumen. () Cohesive epithelial cells with close interdigitations and cilia facing outside. () Phenotypic and structural characteristics of spheroids were maintained up to 8 days. Micrographs are representative of spheroids obtained from COPD and non-COPD patients (n = 11). , ×10; , ×40,, ×80; , ×800; , ×3000; , ×5000; G, ×800. BC, basal cells; CC, ciliated cells; Cil., cilia; arrow indicates cell interdigitations