ZLI formation occurs independently from ventral tissues after stage HH10 and ZLI morphogenesis depends on the integrity of its flanking regions

<p><b>Copyright information:</b></p><p>Taken from "Tissue interactions in the developing chick diencephalon"</p><p>http://www.neuraldevelopment.com/content/2/1/25</p><p>Neural Development 2007;2():25-25.</p><p>Published online 13 Nov 2007</p><p>PMCID:PMC2217525.</p><p></p> Diencephalic explants were taken from stage HH10 and stage HH14 embryos and were analysed by hybridisation for the expression of (a-d, g-j) and (E, F) at the time of dissection (pre-cult.) or after culture for 48 (B, D, J) or 24 hours (F) (post-cult.). (A, B) Explants comprising pro-Pth, pro-ZLI, pro-Th and basal tissue (red). Note basal expression of at the time of dissection and formation of a ZLI after culture (red arrowhead). (C, D) Pro-Pth + pro-ZLI + pro-Th explants excluding basal tissue. Note absence of expression at the time of dissection and induction of a ZLI after culture. (E, F) Explants as in (C, D). Note formation of a -free stripe of cells after culture (red arrowhead). (G, H) Pro-Pth + pro-ZLI explants; (I, J) pro-ZLI + pro-Th explants. Note induction of in a patch, rather than a line, after culture (compare to (B, D))