Is expressed in the dorsal diencephalon and is able to suppress ZLI formation

<p><b>Copyright information:</b></p><p>Taken from "Tissue interactions in the developing chick diencephalon"</p><p>http://www.neuraldevelopment.com/content/2/1/25</p><p>Neural Development 2007;2():25-25.</p><p>Published online 13 Nov 2007</p><p>PMCID:PMC2217525.</p><p></p> Stage HH15 chick head, hybridisation for expression of . Note expression in the dorsoanterior part of the eye, anterior to the MHB and in the epithalamus (red arrowhead). Stage HH16 embryos were electroporated with CYP1B1-IRES-GFP, cultured to stage HH21/22 and analysed by hybridisation for expression of followed by immunochemical detection of green flourescent protein (GFP; lateral views of hemisected brains, anterior to the left, dorsal to the top). (B) Non-electroporated control side (co). (C, D) Electroporated side (ep); (D) an overlay with the anti-GFP fluorescent signal in green. Note reduced expression in the ZLI even in areas where only few cells are transfected (red arrowhead), suggesting a non-autonomous effect