Expression of CCR2 mRNA and protein immunoreactivity in rat lumbar DRG ipsilateral to focal nerve demyelination

<p><b>Copyright information:</b></p><p>Taken from "Delayed functional expression of neuronal chemokine receptors following focal nerve demyelination in the rat: a mechanism for the development of chronic sensitization of peripheral nociceptors"</p><p>http://www.molecularpain.com/content/3/1/38</p><p>Molecular Pain 2007;3():38-38.</p><p>Published online 12 Dec 2007</p><p>PMCID:PMC2228278.</p><p></p> ) Lumbar DRG removed from vehicle-treated animals at POD7 did not exhibit CCR2 mRNA expression (n = 5). ) Many lumbar DRG neurons in vehicle-treated rats sensory neurons were positive for isolectin IB, a neuronal phenotype that distinguishes some C-fiber nociceptors (green cells). There was no evidence of CCR2 protein expression in sham animals (n = 5). ) Lumbar DRG neurons from nerve-injured rats on POD7 exhibited CCR2 mRNA transcripts in some small and medium diameter neurons (black arrows). Open black arrowhead indicates a neuron without CCR2 mRNA transcripts (n = 4). ) Lumbar DRG neurons from a rat subjected to focal nerve demyelination exhibited few CCR2 immunopositive (white arrows) sensory neurons (n = 4). ) Many lumbar DRG neurons on POD14 exhibited CCR2 mRNA transcripts (black arrows). Open arrowhead indicates non-labeled neuron. ) CCR2 immunoreactivity was present in an increased number of neurons at POD14 (white arrows; n = 5). Scale bar is; 30 μm (, ), 50 μm (, , ), and 100 μm ()