Colocalization of IP-10 immunoreactivity (-ir) and isolectin B4 (IB)-binding neurons in the lumbar DRG of naïve rat and rats subjected to LPC-induced nerve injury

<p><b>Copyright information:</b></p><p>Taken from "Delayed functional expression of neuronal chemokine receptors following focal nerve demyelination in the rat: a mechanism for the development of chronic sensitization of peripheral nociceptors"</p><p>http://www.molecularpain.com/content/3/1/38</p><p>Molecular Pain 2007;3():38-38.</p><p>Published online 12 Dec 2007</p><p>PMCID:PMC2228278.</p><p></p> IB-binding in rat DRG neurons distinguishes a population of C-fiber nociceptors. ) The majority of IP-10-ir cells were limited to medium diameter neurons in the lumbar DRG from vehicle-treated rats (red arrows). ) IB-binding small diameter presumptive nociceptors (green arrows) did not colocalize with IP-10-ir lumbar DRG neurons from vehicle-treated rodents at POD7 (, merged images). ) Lumbar DRG ipsilateral to focal nerve demyelination exhibited numerous medium and small diameter IP-10-ir neurons at POD7. Limited numbers of neurons were positive for both IP-10-ir (, red arrows) and IB4-binding (, green arrows) on POD7 (, merged images). ) Many IP-10-ir neurons (red arrows) colocalized with IB-binding neurons (, green arrows) at POD14 (, merged images). Yellow arrows indicate colocalized cells. Scale bar is 100 μm