A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth -3

<p><b>Copyright information:</b></p><p>Taken from "A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth "</p><p>http://www.translational-medicine.com/content/5/1/61</p><p>Journal of Translational Medicine 2007;5():61-61.</p><p>Published online 27 Nov 2007</p><p>PMCID:PMC2235829.</p><p></p> assessed for Annexin V-Alexa Fluor 488 binding by flow cytometry. Cells were counterstained with propidium iodide (PI) to follow non-specific death and the percentage of cells that stained positive for Annexin V and negative for PI was plotted. Representative results from three individual experiments are shown, (A). Only antibody 339.1, which does not cross-react with human α5β1, elicited cell death in these cells. A dose response curve for 339.1 in this assay using SVR murine angiosarcoma cells revealed an ECof 5.3 nM, (A)