Proximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursors-5

<p><b>Copyright information:</b></p><p>Taken from "Proximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursors"</p><p>http://www.biomedcentral.com/1471-213X/7/140</p><p>BMC Developmental Biology 2007;7():140-140.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2231376.</p><p></p>ical removal the VE (-VE) or the ExE (-ExE). (B) E5.75 embryo showing GFP signal in the VE (white arrows). PGC precursors emerge approximately 12 hours later in the proximal epiblast region adjacent to the asterisks. (C, D) Explants derived from E5.75 -VE embryos cultured for 1 day, D1 (C) and 3 days, D3 (D). (E-H) Explants derived from E5.75 whole and -ExE embryos cultured for 1 day, D1 (E, G) and 3 days, D3 (F, H). E, G, white arrows point to Blimp1(GFP)-positive VE; F, white asterisk marks Blimp1(GFP)-positive PGC precursors in the proximal-posterior part of explant; H, red arrow points to Blimp1(GFP)-negative VE, but Blimp1(GFP)-positive epiblast. Cartoons adjacent to the explants elucidate the morphology of the explant. Abbreviations: Emb, tissue derived from epiblast; ExE, extraembryonic ectoderm; thick black line, endoderm. (I, J) Explants derived from E5.75 whole and -ExE (I) and (J) embryos cultured for 3 days, D3. White asterisk marks the proximal-posterior part of explant; -ExE explants were inserted in the lower left corner of I and J. K, After 4 days of culture, the cluster of Blimp1(GFP)-positive cells present in explants from whole embryos turns positive for alkaline phosphatase-activity (AP). Scale bar in B-J is 100 μm and K is 25 μm