Proximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursors-6

<p><b>Copyright information:</b></p><p>Taken from "Proximal visceral endoderm and extraembryonic ectoderm regulate the formation of primordial germ cell precursors"</p><p>http://www.biomedcentral.com/1471-213X/7/140</p><p>BMC Developmental Biology 2007;7():140-140.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2231376.</p><p></p>terisks). (B) After 3 days of culture, the total number of Blimp1(GFP)-positive, Stella-positive and TNAP-positive cells were analysed in each explant derived from E6.75 whole and -VE embryos. Red bars depict the median, n is the number of individual explants analysed. (C-J) Explants derived from E6.75 whole (C, D, G, H) and -VE (E, F, I, J) immunostained to detect Blimp1(GFP) and Stella (C-F), followed by staining to detect alkaline phosphatase-activity (G-J). Asterisks depict PGCs, arrows depict small cells that are (Blimp1)GFP- and TNAP-positive, but Stella negative. Scale bar in A is 100 μm and in C-J is 50 μm