A) A whole cell lysate from (YNH4) cells containing pSMY4 (, ) was centrifuged at 100 000 ×

<p><b>Copyright information:</b></p><p>Taken from "Smy2p Participates in COPII Vesicle Formation Through the Interaction with Sec23p/Sec24p Subcomplex"</p><p></p><p>Traffic (Copenhagen, Denmark) 2007;9(1):79-93.</p><p>Published online 27 Nov 2007</p><p>PMCID:PMC2239301.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> The resulting pellet fraction (mem) was resuspended in sucrose solution and separated on a 20–60% sucrose density gradient. Twelve fractions were collected from the top, resolved by SDS–PAGE and immunoblotted with the anti-HA, anti-Sec24p, anti-Sec31p, anti-Sec61p or anti-Kex2p antibody. Relative amounts of these proteins in each fraction were quantified by densitometry of immunoblots. B) Yeast cells used in A were examined by indirect immunofluorescence with the anti-HA antibody (left panels in the pair). DAPI staining was used to visualize the nuclei (right panels in the pair)