Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells-6

<p><b>Copyright information:</b></p><p>Taken from "Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells"</p><p>http://www.virologyj.com/content/4/1/102</p><p>Virology Journal 2007;4():102-102.</p><p>Published online 23 Oct 2007</p><p>PMCID:PMC2241602.</p><p></p>rectional vector, by replacement of the human pol I promoter sequence in pAD3000 with the MDCK 469 bp sequence upstream of the transcription initiation site. In addition, the two BI restriction sites in pAD3000, which are used for cloning sequences between the two promoters, were changed to I sites because the former restriction site occurs in the MDCK pol I containing fragment