Quantification of total and surface-associated hERG protein in cells maintained at lower temperatures

<p><b>Copyright information:</b></p><p>Taken from "Improved functional expression of recombinant human ether-a-go-go (hERG) Kchannels by cultivation at reduced temperature"</p><p>http://www.biomedcentral.com/1472-6750/7/93</p><p>BMC Biotechnology 2007;7():93-93.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2241608.</p><p></p> Cells were cultured for 3 d and analyzed by flow cytometry. Data were averaged from 6 samples, each of 5000 cells (un-gated), and normalized against CHO hERG at 37°C. Normalized fluorescence intensity of fixed and permeablized CHO hERG (open bars) and untransfected control CHO cells (filled bars) at the respective temperatures stained with antibody C20 (which is raised against a C-terminal peptide sequence; A). Representative population fluorescence plots for CHO hERG cells from 37, 30 and 27°C in the same experiment as A (B). Normalized surface fluorescence intensity of non-fixed and non-permeablised CHO hERG (open bars) and control cells (filled bars) maintained at the respective temperatures stained with antibody 2110 (which is raised against a peptide between TM1 and TM2 which is predicted to be located on the surface of the plasma membrane; C)