The effect of deletion of the orphan G – protein coupled receptor (GPCR) gene on pain-like behaviours in mice-0

<p><b>Copyright information:</b></p><p>Taken from "The effect of deletion of the orphan G – protein coupled receptor (GPCR) gene on pain-like behaviours in mice"</p><p>http://www.molecularpain.com/content/4/1/2</p><p>Molecular Pain 2008;4():2-2.</p><p>Published online 15 Jan 2008</p><p>PMCID:PMC2242784.</p><p></p>tal of 776 base pairs from within the 930 base pair coding region located in exon 2 of the 2 exon gene (first exon is un-translated) was deleted and replaced with a 7,076 base pair IRES-lacZ reporter and neomycin resistance cassette. Solid Line: intron 1; boxes colored red: exons 1 and 2; box colored blue: coding sequence located in exon 2; uncoloured box: IRES-LacZ-NEO cassette replacing part of the coding sequence located in exon 2; black triangle: FRT sequence. (B) Genotyping assay was designed to differentiate among wild type (+/+), heterozygote (+/-) or homozygote knockout (-/-) mice and performed using a multiplex PCR reaction, with a shared 5' forward primer (5'-GCA GAC ATC AGC CAT GAC GT-3') and a 3' reverse primer unique to the targeted allele (#2416, 5'-ATC AGC TTA CCA TGG CCA AGA TCC C-3') or to the locus (5'-ATC TAT CTC TTG GAT GTG GCC TG-3'). The WT amplicon is 201 and KO allele 922 bps