Single-cell duplex RT-LATE-PCR reveals and RNA gradients in 8-cell embryos-0

<p><b>Copyright information:</b></p><p>Taken from "Single-cell duplex RT-LATE-PCR reveals and RNA gradients in 8-cell embryos"</p><p>http://www.biomedcentral.com/1472-6750/7/87</p><p>BMC Biotechnology 2007;7():87-87.</p><p>Published online 7 Dec 2007</p><p>PMCID:PMC2246118.</p><p></p>xpanded blastocyst after RT (blue line, RNA + DNA), expanded blastocyst without RT (pink line, DNA only) and single TE cell after RT (green line, RNA + DNA). Whole blastocysts contain RNA (as shown by the delta between the pink and the blue line) due to the presence of the ICM. expression is downregulated in the TE (the green line crosses the threshold very close to the 2-copy marker; two template copies per cell are due to the presence of genomic DNA). The positions of the white-to-red arrows correspond to known copy numbers in a genomic DNA standard, as color-coded; the placement of the green arrow was calculated based on this standard. Inset: expression in the ICM isolated from an early blastocyst. Half of the ICM was used for the "+RT" determination (blue line) and half was used for the "No RT" determination (pink line). The probe used in all these experiments was an -specific molecular beacon conjugated to TET