Single-cell duplex RT-LATE-PCR reveals and RNA gradients in 8-cell embryos-4

<p><b>Copyright information:</b></p><p>Taken from "Single-cell duplex RT-LATE-PCR reveals and RNA gradients in 8-cell embryos"</p><p>http://www.biomedcentral.com/1472-6750/7/87</p><p>BMC Biotechnology 2007;7():87-87.</p><p>Published online 7 Dec 2007</p><p>PMCID:PMC2246118.</p><p></p>eight female embryos at the 8-cell stage. Genomic DNA amounts to two copies in each cell and is negligible on the scale used for this graph. Bars of the same color indicate cells extracted from the same embryo, which were arranged and numbered in order of increasing RNA content. When fewer than eight cells were recovered, the collected blastomeres were assigned to the groups with the closest RNA contents. (B) RNA+DNA levels in individual blastomeres recovered from fourteen male and female 8-cell embryos. Two copies of template per cell are due to the presence of genomic DNA (negligible on the scale used). Bars were arranged with the same criteria used for expression analysis in panel A; colors were used independently in the two panels. By the 8-cell stage blastomeres comprising the same embryo consistently exhibit different levels of expression of both the and the gene