DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines-0

<p><b>Copyright information:</b></p><p>Taken from "DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines"</p><p>http://www.molecular-cancer.com/content/7/1/15</p><p>Molecular Cancer 2008;7():15-15.</p><p>Published online 25 Jan 2008</p><p>PMCID:PMC2246151.</p><p></p>re shown. U = unmethylated MSP product, M = methylated MSP product. Cell line abbreviations are as follows: 231 = MDA-MB-231, 415 = MDA-MB-415, 435S = MDA-MB-435S, 436 = MDA-MB-436, 453 = MDA-MB-453, and 468 = MDA-MB-468. All other cell lines are designated by their full name. (B) Representative bisulfite sequence analysis for . Methylated CpGs are designated by closed circles, unmethylated CpGs are designated by open circles for MDA-MB-435S, BT20, and MDA-MB-231 cell lines (5 replicates each). (C) Representative agarose gels of RT-PCR products for , and demonstrating 5-aza induction of gene expression in hypermethylator cell lines. RT-PCR results using cDNA template from untreated (-) and 5-aza treated (+) are shown