Cytotoxicity of NK cells after proliferation in the presence of cytokines

<p><b>Copyright information:</b></p><p>Taken from "The proinflammatory cytokines IL-2, IL-15 and IL-21 modulate the repertoire of mature human natural killer cell receptors"</p><p>http://arthritis-research.com/content/9/6/R125</p><p>Arthritis Research & Therapy 2007;9(6):R125-R125.</p><p>Published online 3 Dec 2007</p><p>PMCID:PMC2246246.</p><p></p> Cytotoxicity of natural killer (NK) cells to K562 cells. Human primary NK cells were purified by magnetic beads and cultured for 7 days with IL-2 or IL-15 in the presence or absence of IL-21. Both populations were analyzed for cytoxicity to K562 target cells at ratios of 10:1 and 1:1. One of three similar experiments is shown. Cytotoxicity of NKp44-positive and NKp44-negative NK cells. Human primary NK cells were purified by magnetic beads (bulk) and cultured for 7 days with IL-15 and IL-21. The NKp44-positive and NKp44-negative subpopulations were sorted by fluorescence-activated cell sorting and were analyzed for their cytotoxicity to K562 targets cells at ratios of 10:1 and 1:1. One of three similar experiments is shown