Identification of chromosomal alpha-proteobacterial small RNAs by comparative genome analysis and detection in strain 1021-0

<p><b>Copyright information:</b></p><p>Taken from "Identification of chromosomal alpha-proteobacterial small RNAs by comparative genome analysis and detection in strain 1021"</p><p>http://www.biomedcentral.com/1471-2164/8/467</p><p>BMC Genomics 2007;8():467-467.</p><p>Published online 19 Dec 2007</p><p>PMCID:PMC2245857.</p><p></p>f total RNA from strain 1021 was analyzed by Northern blotting hybridization with specific oligonucleotides [see Additional file ]; the molecular sizes were calculated in nucleotides (nt): > rnpB (372 nt), > tmRNA 3' end (204 nt), > 5S (120 nt), > 4.5S (95 nt), and > tmRNA 5' end (82 nt) [39]. Band > was not hybridized but its size is consistent with it being a tRNA. Exposure times were optimized for each panel and signal intensity does not indicate relative abundance of ncRNAs