Post-translational modifications of histones H3 and H4 associated with the chromatin-binding proteins Suv39h1 and G9a

<p><b>Copyright information:</b></p><p>Taken from "Post-translational modifications of histones H3 and H4 associated with the histone methyltransferases Suv39h1 and G9a"</p><p>http://genomebiology.com/2007/8/12/R270</p><p>Genome Biology 2007;8(12):R270-R270.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2246272.</p><p></p> Schematic representation of the purification protocols used to purify the HMT-histone complexes and crude histones. Doubly immunopurified Suv39h1 complexes from chromatin extracts of 20 g of HeLa-Suv39h1 cells were resolved on a 4-12% gradient NuPAGE gel, run in MES buffer (Invitrogen), fixed, and stained with Colloidal blue. Lane 1, SeeBlue pre-stained molecular weight markers (Invitrogen); lane 2, Suv39h1 complex from chromatin fractions. Amino-terminal lysine methylation of histones H3 and H4 associated with Suv39h1 or G9a proteins. Post-translational modifications of histone H4 associated with Suv39h1 or G9a. Shown are the means of three independent experiments (± standard deviation)