TGZ suppresses TGFβ2-induced expression of fibronectin and collagen type I in subconfluent ARPE-19 cells

<p><b>Copyright information:</b></p><p>Taken from "Troglitazone suppresses transforming growth factor beta-mediated fibrogenesis in retinal pigment epithelial cells"</p><p></p><p>Molecular Vision 2008;14():95-104.</p><p>Published online 18 Jan 2008</p><p>PMCID:PMC2254970.</p><p></p>ARPE-19 cells at subconfluent density of 5 X 10 cells per well in six-well plates were either left untreated (UT) or were treated with 4 ng/ml TGFβ2, or were pretreated with different doses of TGZ (1, 5, 10 μM; 1 h) or DMSO (0.05%, 1 h) and then incubated with TGFβ2. The expressions of fibronectin and collagen type I were assayed by RT–PCR analysis (A), western blot analysis (B), and ELISA (C) as – described. *