Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters-4

S and supernatants were harvested daily. Total RNA was extracted, and 7 replicate aliquots were separated on 1% agarose gel in the presence of 0.44 M formaldehyde gel, transferred to a nylon membrane and hybridized with digoxigenin-labeled single-stranded anti-sense riboprobes to detect mRNA as follows: A) M2 riboprobe; B) SH riboprobe; C) N riboprobe; D) F riboprobe; E) G riboprobe. F) Sense P, M, and F riboprobes were combined to detect genomic RNA. G) RNA in a duplicate gel was visualized with ethidium bromide and photographed under UV light. H) Titers of samples prior to RNA extraction were determined by plaque assay in Vero cells.<p><b>Copyright information:</b></p><p>Taken from "Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters"</p><p>http://www.virologyj.com/content/5/1/69</p><p>Virology Journal 2008;5():69-69.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2426676.</p><p>