The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish-0

) in the trunk and tail of 24 hpf zebrafish embryos. Notice that U0126-treated embryos have been overexposed (can be judged by the autofluorescence of the yolk sac extension) and still fail to show specific staining. : U0124 does not eliminate the p-Erk staining. : PD98059 also does not eliminate p-Erk staining when given just below the level of lethal toxicity (25 μM). : Somewhat weaker but still significant p-Erk staining in embryos treated with U0126 starting at 10 hpf. : Western-blot analysis demonstrated a slight but incomplete reduction in pErk1/2 levels in 10-somite embryos.: Stage and dose-dependency of U0126 application. There is a dramatic drop in the percentage of affected embryos when treatment is applied after epiboly is completed. : Washout experiments reveal the necessity of U0126 to be present until at least the 18 somite stage to produce the full phenotype.<p><b>Copyright information:</b></p><p>Taken from "The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish"</p><p>http://www.biomedcentral.com/1471-213X/8/42</p><p>BMC Developmental Biology 2008;8():42-42.</p><p>Published online 17 Apr 2008</p><p>PMCID:PMC2359734.</p><p>