Early antibody response against subspecies antigens in subclinical cattle-3

E left margin and blotted onto nitrocellulose filters. The filters were placed into a slot blotting device and 1:500 dilutions of each cattle sera were loaded into independent slots. Each blot was further processed as described previously [29]. The positive control, indicated by the lane with a "+" sign above it, was probed with a monoclonal antibody developed against the MBP affinity tag as described previously [29]. These results demonstrate that MAP1087 and MAP1204 are detected by sera from naturally infected cattle in the subclinical phase of infection. Serum sample slot assignments: 1, cow 85; 2, cow 112; 3, cow 113; 4, cow 117; 5, cow 183; 6, cow 235; 7, cow 834; 8, cow 871 and 9, cow 2715.<p><b>Copyright information:</b></p><p>Taken from "Early antibody response against subspecies antigens in subclinical cattle"</p><p>http://www.proteomesci.com/content/6/1/5</p><p>Proteome Science 2008;6():5-5.</p><p>Published online 28 Jan 2008</p><p>PMCID:PMC2265687.</p><p>