The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues-2

5) in combination with hSIN3B (S) as described in Materials and Methods. IP and Western were performed with α-V5 (V5 specific) and α-S (hSIN3B specific). () ETO and MTG16 co-precipitated hSIN3B (Lanes 1 and 7 of A respectively). Reciprocal experiments showed that hSIN3B co-precipitated ETO (Lanes 2 and 8 of B respectively). Arrowhead shows the position of ETO in A and MTGR1 in B. MTGR1 failed to co-precipitate hSIN3B (Lane 4 of A). The reciprocal experiment showed that hSIN3B did not precipitate MTGR1 (Lane 5 of B). Lower panels in A and B show input of hSIN3B and ETO homologue in 2% of IP lysate. The positions of the molecular weight markers are indicated at the left.<p><b>Copyright information:</b></p><p>Taken from "The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues"</p><p>http://www.biomedcentral.com/1471-2199/9/8</p><p>BMC Molecular Biology 2008;9():8-8.</p><p>Published online 19 Jan 2008</p><p>PMCID:PMC2266940.</p><p>