Fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transcriptase-2

M-Vpr-X4-tropic NL4-3, or (C) infected in the presence of 10 μg/ml SP4-2, or (D) infected in the presence of 250 nM AMD3100. In a parallel experiment, SupT1 cells (1 × 10) were either (E) mock infected, or (F) infected for 2 h at 0.5 moi with BlaM-Vpr-X4-tropic NL4-3, or (G) infected in the presence of 20 ng/ml sCD4, or (H) infected in the presence of 20 ng/ml sCD4 together with 16 μg/ml SP4-2. Cells were loaded with CCF2/AM dye and fusion was analyzed by multiparameter flow cytometry using a violet laser for excitation of CCF, and gated from 10,000 cells. Percentages in each panel are of cells displaying blue fluorescence (virus fusion positive cells). Representative of 3 separate experiments.<p><b>Copyright information:</b></p><p>Taken from "fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transcriptase"</p><p>http://www.virologyj.com/content/5/1/8</p><p>Virology Journal 2008;5():8-8.</p><p>Published online 15 Jan 2008</p><p>PMCID:PMC2267448.</p><p>