Fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transcriptase-5

10M ddC, or mock treated (0 μg SP4-2), as indicated. Then, cells were infected with HIV-1 (NL4-3) at multiplicity of infection (moi) of 0.01 for 1.5 h, washed 3 times, and returned to culture with the same concentration of each treatment, for the duration of the experiment. (A) On day 3 after infection, HIV-1 infection was quantified by luciferase gene marker expression from cell lysates that were normalized to the same number of viable cells, and expressed as relative light units (RLU) on the y-axis. (B) Viability for each cell culture treatment was quantified by MTT uptake. (C) Percent inhibition of HIV-1 was calculated from raw data in (A), utilizing the formula in the Methods, and plotted on the Y-axis as % HIV-1 Inhibition. Data are mean ± SD of three separate experiments.<p><b>Copyright information:</b></p><p>Taken from "fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transcriptase"</p><p>http://www.virologyj.com/content/5/1/8</p><p>Virology Journal 2008;5():8-8.</p><p>Published online 15 Jan 2008</p><p>PMCID:PMC2267448.</p><p>