Compartments labelled by positively charged nanogold during time-course experiments

(A, B) Labelled MVB fusing with the vacuole (*). The particles were seen in MVBs (see arrows). (C) No nanogold staining was observed in systems of interconnected vesicles forming a single compartment after 15 min of incubation in time-course experiments (arrow: interconnections). (D) Labelling appeared in these compartments after 30 min of incubation with positively charged nanogold and gold particles appeared to detach from the membrane (see arrows). Bar=100 nm.<p><b>Copyright information:</b></p><p>Taken from "Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3051-3068.</p><p>Published online 4 Jul 2008</p><p>PMCID:PMC2504345.</p><p>