(a) HeLa cells incubated with RNAi against Cα and Cβ and monitored for PKA-specific phosphotransferase activity in cell extracts of 1 mg/mL

Relative values given as counts per minute (cpm). (b) Relative EGFR mRNA expression levels were measured by RT-PCR and are given as E/G × 100 where E and G are the relative copy numbers of EGFR and GAPDH mRNA, respectively. The results are given as +/- SEM where n = 3. (c) Western immunoblotting analysis of expression of EGFR and PKA C. An anti-actin antibody was applied to ensure equal loading.<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit"</p><p>http://www.biomedcentral.com/1471-2121/9/16</p><p>BMC Cell Biology 2008;9():16-16.</p><p>Published online 1 Apr 2008</p><p>PMCID:PMC2324083.</p><p>