Flow chart of the SIMPLE approach used to identify mutants expressing StdA

The bottom panel shows a Western blot detected with rabbit anti-StdA serum. Each lane represents a culture inoculated with a single colony from a mutant pool after two rounds of enrichment with immuno-magnetic particles coated with anti-StdA serum. The molecular mass of standard proteins is indicated on the right.<p><b>Copyright information:</b></p><p>Taken from "RosE represses Std fimbrial expression in serotype Typhimurium"</p><p></p><p>Molecular Microbiology 2008;68(3):573-587.</p><p>Published online 01 May 2008</p><p>PMCID:PMC2328253.</p><p>© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd