Uri, a PP1α binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity-5

Ingle channel greyscale images of the boxed region are shown in A, B, and the merge of these is shown in C. Uri co-localises with active RNAPII on normal larval polytene chromosomes (A-C; overlap is yellow in C.). D-F, After heat shock, RNAPII activity is restricted to the heat-shock puffs (E), Uri co-localises to these puffs (D, F). G, RT-PCR of potential target genes from homozygous embryos and /sibling control embryos. The results of three independent experiments are shown; size marker is shown on the left. mRNA levels of and varied somewhat between experiments; was slightly reduced in mutant compared to control embryos, on average was equal in mutant compared to control. and mRNA levels were significantly and reproducibly lower in the mutant embryos than in control embryos. GFP control primers confirm the accuracy of embryo selection based on fluorescence. Asterisks indicate primer dimer bands.<p><b>Copyright information:</b></p><p>Taken from "Uri, a PP1α binding protein, is essential for viability, maintenance of DNA integrity and normal transcriptional activity"</p><p>http://www.biomedcentral.com/1471-2199/9/36</p><p>BMC Molecular Biology 2008;9():36-36.</p><p>Published online 15 Apr 2008</p><p>PMCID:PMC2346476.</p><p>