IAlignments of target (A gene segments) and probe sequences are shown on the left-hand side, and the respective hybridization signals (including internal staining marker) are given on the right-hand side

Upper part: The signal generated by duplex formation at genotype E-specific probe VD2-04 is reduced to approx. 20% when the target has a single mismatch, such as genotype B. This applies also to genotype B-specific probe VD2-03, when reacting with genotype E. Lower part: Signal is reduced to less than 10% in the case of two mismatches on the target sequence.<p><b>Copyright information:</b></p><p>Taken from "Genotyping of using a new DNA microarray assay based on sequence analysis of A genes"</p><p>http://www.biomedcentral.com/1471-2180/8/63</p><p>BMC Microbiology 2008;8():63-63.</p><p>Published online 17 Apr 2008</p><p>PMCID:PMC2362127.</p><p>