T98G (B) or IMR-90 (C) cells were synchronized by serum deprivation for 72 h and then induced to cycle by refeeding with 10% FBS. Aliquots of cells were harvested at the times indicated and assayed for Rac1 and the indicated control proteins by immunoblotting. Bar, 5 μm.<p><b>Copyright information:</b></p><p>Taken from "Rac1 accumulates in the nucleus during the G2 phase of the cell cycle and promotes cell division"</p><p></p><p>The Journal of Cell Biology 2008;181(3):485-496.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2364699.</p><p></p
<p>(A) Immunofluorescent images of MC3T3-E1 cells transfected with Rab1-GFP overnight and differenti...
<p>(A) MDCK cells expressing Raichu-Rac1 were cultured to form cysts for 10 days, treated with HGF f...
<p><b>A</b> representative single cell image series of Rvs167-GFP distribution at different stages o...
The percentage of cells with nuclear Rac1 was determined hourly and plotted as mean ± SEM ( = 4). (B...
S1, available at ) were scored for the percentage of cells showing strong nuclear fluorescence befor...
Arrowheads indicate representative parent and daughter cells. Note that nuclear Rac1 is high immedia...
Rac1 regulates a wide variety of cellular processes. The polybasic region of the Rac1 C terminus fun...
<p>(<b>A</b>) HL-7702 cells transfected with an empty control or Myc-RAD6 plasmids were stained with...
<p>A) HeLa cells stably expressing GFP-Rac1 were transfected with scrambled or Rab5 isoform siRNAs. ...
(A, B) RAD5-Myc cells grown to early log phase at 30°C were arrested in G1 with α-factor for 2 h. Ce...
<p>(A) The level of active Rac1-GTP was monitored in MCF-7 cells expressing different SDC4 construct...
<p>The cell cycle distribution of the cells was analyzed by flow cytometry. The representative graph...
<p>A. NSCLC cells were serum starved for 24 h and then incubated with 10% FBS (5 min). Rac-GTP level...
<p>(A) Rac1 GTP loading was measured by biotinylated Pak-CRIB peptide-based pull-down (PD) with lysa...
<p>(A) MDA-MB-231 cells were transfected with the indicated siRNAs and the levels of active Rac were...
<p>(A) Immunofluorescent images of MC3T3-E1 cells transfected with Rab1-GFP overnight and differenti...
<p>(A) MDCK cells expressing Raichu-Rac1 were cultured to form cysts for 10 days, treated with HGF f...
<p><b>A</b> representative single cell image series of Rvs167-GFP distribution at different stages o...
The percentage of cells with nuclear Rac1 was determined hourly and plotted as mean ± SEM ( = 4). (B...
S1, available at ) were scored for the percentage of cells showing strong nuclear fluorescence befor...
Arrowheads indicate representative parent and daughter cells. Note that nuclear Rac1 is high immedia...
Rac1 regulates a wide variety of cellular processes. The polybasic region of the Rac1 C terminus fun...
<p>(<b>A</b>) HL-7702 cells transfected with an empty control or Myc-RAD6 plasmids were stained with...
<p>A) HeLa cells stably expressing GFP-Rac1 were transfected with scrambled or Rab5 isoform siRNAs. ...
(A, B) RAD5-Myc cells grown to early log phase at 30°C were arrested in G1 with α-factor for 2 h. Ce...
<p>(A) The level of active Rac1-GTP was monitored in MCF-7 cells expressing different SDC4 construct...
<p>The cell cycle distribution of the cells was analyzed by flow cytometry. The representative graph...
<p>A. NSCLC cells were serum starved for 24 h and then incubated with 10% FBS (5 min). Rac-GTP level...
<p>(A) Rac1 GTP loading was measured by biotinylated Pak-CRIB peptide-based pull-down (PD) with lysa...
<p>(A) MDA-MB-231 cells were transfected with the indicated siRNAs and the levels of active Rac were...
<p>(A) Immunofluorescent images of MC3T3-E1 cells transfected with Rab1-GFP overnight and differenti...
<p>(A) MDCK cells expressing Raichu-Rac1 were cultured to form cysts for 10 days, treated with HGF f...
<p><b>A</b> representative single cell image series of Rvs167-GFP distribution at different stages o...