(A) Lin blood cells of heterozygous CCR9-EGFP knock-in mice were fractionated according to their CD117 and CCR9 expression

The plot is gated on Lin cells. Percentages are indicated. (B) Lin fractions with the indicated phenotypes were isolated from blood (as shown in A) and from bone marrow, and were cultured as groups of 100 cells on OP9-DL1 stromal layers for 12 and 19 d. The fraction of wells that contained T lineage–committed DN3/4 thymocytes is plotted for each time point. At least 1,200 cells cultured as 100 cells per well were assayed for each population and time point. For the analysis of T lineage precursor activity of Lin CD117 CCR9 blood cells at day 12, >2,000 cells cultured as 100 cells per well were analyzed.<p><b>Copyright information:</b></p><p>Taken from "The stream of precursors that colonizes the thymus proceeds selectively through the early T lineage precursor stage of T cell development"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1187-1199.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373849.</p><p>