Validation of gene expression of stimulated chondrocytes by real-time RT-PCR

Semi-quantitative real-time RT-PCR of selected genes that were differentially expressed in chondrocytes stimulated with supernatant of a synovial fibroblast cell line derived from a patient with rheumatoid arthritis (RASFsn) as determined by microarray analysis was performed. Real-time RT-PCR gene expression analysis determined that the expression of (), () and () was significantly induced during stimulation of cartilage-like cultures with RASFsn compared with stimulation with supernatant of a synovial fibroblast cell line derived from normal donor (NDSFsn). The gene expression of (), (), () and () was significantly repressed during stimulation with RASFsn. Consistent changes were observed between real-time RT-PCR and microarray analysis for all genes examined. The expression of selected genes was calculated as the percentage of () expression. The mean of each triplicate well of both experimental groups is plotted and the error bars represent SD. For statistical analysis, Students t-test was applied (*, ≤ 0.05; ***, ≤ 0.001).<p><b>Copyright information:</b></p><p>Taken from "Key regulatory molecules of cartilage destruction in rheumatoid arthritis: an study"</p><p>http://arthritis-research.com/content/10/1/R9</p><p>Arthritis Research & Therapy 2008;10(1):R9-R9.</p><p>Published online 18 Jan 2008</p><p>PMCID:PMC2374452.</p><p>