Fibrinogen decreases cardiomyocyte contractility through an ICAM-1-dependent mechanism-2

1). Isolated cardiomyocytes were incubated with Oregon Green-labeled fibrinogen (green) and fluorescently stained for ICAM-1 (red). A multiphoton dual-excitation image of the contour of the cell of interest is shown. By means of an overlay of images, strong colocalization of fibrinogen and ICAM-1 was indicated by a yellow color. A representative image of a rat cardiomyocyte with adherent fibrinogen-coated polystyrene beads (white arrows) is shown to the left of the graph. The specificity of the ICAM-1-fibrinogen interaction is demonstrated as anti-ICAM-1 antibody pre-treatment results in significantly less fibrinogen-coated polystyrene beads adherent to the cardiomyocytes (*< 0.05 versus control). Ab, antibody; CL, control; IgG, immunoglobulin G.<p><b>Copyright information:</b></p><p>Taken from "Fibrinogen decreases cardiomyocyte contractility through an ICAM-1-dependent mechanism"</p><p>http://ccforum.com/content/12/1/R2</p><p>Critical Care 2008;12(1):R2-R2.</p><p>Published online 3 Jan 2008</p><p>PMCID:PMC2374637.</p><p>