Wild-type strains of , and were grown in MOPS glucose plus nicotinate or MOPS glucose defined-rich media

The data are from two independent experiments (open and closed symbols). Growth curves (, MOPS glucose medium; , MOPS rich medium) and Lrp protein levels (, glucose; , rich) are shown. Equal amounts of total protein were loaded in each lane, and a standard curve of purified Lrp was included on each gel for quantitation. shows a comparative western blot. Cell pellets were boiled and equal amounts of total protein from (Ec) and (Pm) were resolved side-by-side via SDS polyacrylamide gel electrophoresis. The subsequent blot was probed with polyclonal antiserum raised against Lrp.<p><b>Copyright information:</b></p><p>Taken from "Limited functional conservation of a global regulator among related bacterial genera: Lrp in , and "</p><p>http://www.biomedcentral.com/1471-2180/8/60</p><p>BMC Microbiology 2008;8():60-60.</p><p>Published online 11 Apr 2008</p><p>PMCID:PMC2374795.</p><p>