Limited functional conservation of a global regulator among related bacterial genera: Lrp in , and -7

Ng standard errors. At least four points from each of two independent experiments were used to generate each plotted value. Conditions were MOPS-glucose minimal medium, supplemented as described in Methods, in logarithmic (MinLog) or stationary phase (MinSta), or MOPS glucose defined rich medium in those growth phases (RchLog, RchSta). . Direct comparison of mRNA levels.. A baseline amount of total RNA (from a mid-log phase culture in MOPS glucose plus nicotinate) was mixed with varying amounts of test RNA (all from log-phase cultures) from glucose (open circle) or rich (closed circle) cultures. The mixes were used as template for simultaneous amplification with three primer pairs. If the test cDNA preparation has the same proportion of cDNA as the reference pool, the detected amount of cDNA should rise with a slope of 1.0 (actual . detected, based on the varied amounts of test cDNA added); this is shown as a dotted line in each panel.<p><b>Copyright information:</b></p><p>Taken from "Limited functional conservation of a global regulator among related bacterial genera: Lrp in , and "</p><p>http://www.biomedcentral.com/1471-2180/8/60</p><p>BMC Microbiology 2008;8():60-60.</p><p>Published online 11 Apr 2008</p><p>PMCID:PMC2374795.</p><p>