Expression of NFAT1 was inhibited through the PI3K/Akt pathway in GM3S-knockdown 4T1 cells

Upper panels: expression of NFAT1 was detected by immunoblotting. Lower panels: quantitation of these blots after normalization with the GAPDH blot. Upper panels: expression of NFAT1 was enhanced by the PI3K inhibitor LY294002. Lower panels: quantitation of these blots after normalization with the GAPDH blot. *< 0.05, **< 0.01. CMP, cytidine 5'-monophosphate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GM3S, CMP-N-acetylneuraminic acid:lactosylceramide 2,3-sialyltransferase; NFAT, nuclear factor of activated T cell; PI3K, phosphoinositide-3 kinase.<p><b>Copyright information:</b></p><p>Taken from "Silencing of GM3 synthase suppresses lung metastasis of murine breast cancer cells"</p><p>http://breast-cancer-research.com/content/10/1/R1</p><p>Breast Cancer Research : BCR 2008;10(1):R1-R1.</p><p>Published online 3 Jan 2008</p><p>PMCID:PMC2374951.</p><p>