FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in -6

Umber of recombination events between the loci in three-factor crosses. From trans-heterozygotes, 30/34 Dpy-non-Unc recombinants harbored and 10/99 Unc-non-Dpy recombinants harbored . The estimated genetic distance between and is 0.22 map units. (B) A diagram of the cosmid B0523. Genes on the cosmid are indicated as arrows. (C) The gene. 5' is to the left. Black boxes indicate coding exons, and white boxes represent non-coding exons. The extent of the deletion is indicated below the line, as is the location of a Trp tRNA gene. The white box above the line indicates the region used in RNAi experiments, and the black line represents 1 kb. The gene structure is derived from a cDNA previously described (Genbank accession number ). (D) A diagram of the predicted FLI-1 polypeptide. The locations of the leucine-rich repeats (LRRs) and the five gelsolin-like repeats (G1–G5) are indicated. The deletion removes coding region for the residues of FLI-1 indicated below the diagram. (E) A homozygous embryo arrested before embryonic elongation. (F) A homozygous mutant embryo arrested at the two-fold stage and showed a paralyzed arrest at two-fold (Pat) phenotype. The scale bar in (E) applies to (E) and (F).<p><b>Copyright information:</b></p><p>Taken from "FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in "</p><p>http://www.biomedcentral.com/1471-213X/8/54</p><p>BMC Developmental Biology 2008;8():54-54.</p><p>Published online 16 May 2008</p><p>PMCID:PMC2396608.</p><p>