(A) Representative myocardial cryosections from 3-mo-old WT and MHCsTNF littermates were labeled for desmin and analyzed by confocal microscopy

Nuclei were stained with DAPI. (B) Electron micrographs from 3-mo-old WT and MHCsTNF mice. Arrows in WT point to IDs. Perinuclear aggregates and sarcoplasmic aggregates are displayed in TNF 2 and 1, respectively. Aggregates are outlined with dashed lines. TNF 3 is an enlargement of TNF 2 showing Z disks adjacent to an aggregate (asterisk) with material apparently merging into the aggregate (arrowheads). The white arrow in the inset of TNF 3 shows aggregates with electron-dense material. n, nucleus. Bars: (A) 20 μm; (B, top and bottom left) 5 μm; (B, bottom right) 2 μm.<p><b>Copyright information:</b></p><p>Taken from "Desmin mediates TNF-α–induced aggregate formation and intercalated disk reorganization in heart failure"</p><p></p><p>The Journal of Cell Biology 2008;181(5):761-775.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2396798.</p><p>